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For Beginners: The ProteoShell Kit I

Our starter Kit contains everything you need to test the expression of  your proteins using the ProteoShell system.

Note: We have reviewed our product catalogue, pricing, and Terms and Conditions (22 Jan 2024).
We offer discounts up to 60% for academics and first-time customers: contact us at info@lockandkeybiosciences.com to get a promotion code.

The ProteoShell Kit I is designed to boost recombinant protein expression in E. coli. It is simple, fast, and effective. You will only require common laboratory equipment and skills to use it. Key features: •By inserting your gene into the pPS-I plasmid, your protein is merged N-terminally with ProteoShell •ProteoShells are expressed in T7 E. coli strains, e.g. BL21 (DE3) and derivatives •Induction is regulated with lactose / IPTG •Simple isolation of ProteoShells via selective precipitation followed by resolubilization •Release of the protein of interest using TEV protease (not included) •Optimal results for non-membrane proteins (or soluble domains of membrane proteins) with domains > 50KDa Kit contents: •pPS-I-(a) expression plasmid •Lysis Buffer •Harvest Buffer •4x Washing Buffer •4x Reconstitution Buffer The Kit is offered with reagents sufficient for 10 or 50 protein preparations. A preparation is defined as the processing of 10 g of freshly harvested cells, although the procedure is readily adapted to any desired amount.

For Advanced Users: The ProteoShell Kit I plus

Our expert users choose this Kit to tune the amount of protein of interest contained in each ProteoShell. Ideal for very small or very large proteins, and slow-translating sequences.

Note: We have reviewed our product catalogue, pricing, and Terms and Conditions (22 Jan 2024).
We offer discounts up to 60% for academics and first-time customers: contact us at info@lockandkeybiosciences.com to get a promotion code.

The ProteoShell Kit I is designed to boost recombinant protein expression in E. coli. It is simple, fast, and effective. You will only require common laboratory equipment and skills to use it. Key features: •By inserting your gene into the pPS-I plasmid, your protein is merged N-terminally with ProteoShell •Choose among the three pPS-I plasmids (a, b, and c) to tune the amount of protein of interest contained in each ProteoShell •ProteoShells are expressed in T7 E. coli strains, e.g. BL21 (DE3) and derivatives •Induction is regulated with lactose / IPTG •Simple isolation of ProteoShells via selective precipitation followed by resolubilization •Release of the protein of interest using TEV protease (not included) •Optimal results for non-membrane proteins (or soluble domains of membrane proteins) with domains > 50KDa Kit contents: •Expression plasmids pPS-I-(a), pPS-I-(b), and pPS-I-(c) •Lysis Buffer •Harvest Buffer •4x Washing Buffer •4x Reconstitution Buffer The Kit is offered with reagents sufficient for 10 or 50 protein preparations. A preparation is defined as the processing of 10 g of freshly harvested cells, although the procedure is readily adapted to any desired amount.

For Industrial Customers and Partners: The ProteoShell Kit I developer package

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The ProteoShell Technology is applicable to the industrial production of proteins. To this end, we offer you the opportunity to access confidential information under agreement develop your own custom ProteoShell applications. Contact us for more information.

Terms and Conditions (updated 22/01/2024)

These Terms and Conditions (hereafter "Terms") govern the purchase and use of products and materials (hereafter "Products") provided by Lock and Key Biosciences GmbH (hereafter "LKB"). "Customer" refers to any company or institution acquiring Products from LKB.

1. Intellectual Property

Customer acknowledges that all intellectual property rights related to Products are the exclusive property of LKB, including but not limited to patents, trademarks, copyrights, and trade secrets. The purchase of LKB's Products grants the Customer a limited, non-transferable right to use the quantity of purchased Product in accordance with these Terms and to utilize the Product documentation and content. The purchase of Products does not confer any license rights to LKB's intellectual property.

2. Limited Use

By acquiring a Product, the Customer gains a non-transferable right to use the Products exclusively for in-house research purposes within the Customer's current professional venue. The Customer is responsible for adhering to the corresponding instructions and safety precautions, as well as all applicable laws and regulations. Components of the Products may not be used separately or outside the scope of use and the instructions provided with the respective Product.

The Customer may not share or sell any Product components or derivatives. Using Products to create a commercial product requires LKB's approval and is subject to a separate royalty-bearing license agreement. Using Products to produce data or information that is sold, publicly disclosed, or submitted to authorities requires LKB's approval and is subject to a separate royalty-bearing license agreement. Customer is prohibited from studying, reverse-engineering, disclosing, or sharing the composition or sequence of any Product components. Additionally, Customer may not copy or share any information contained in the manuals and other materials obtained with the Products. LKB disclaims responsibility or liability for any injury or damage resulting from the Customer's failure to use Products in accordance with these conditions. Non-compliance with this section of the Terms will result in the termination of the Customer's right to use the Products. The Customer is liable for any losses incurred by LKB due to non-compliance with this section of the Terms.

One year after the purchase of a Product, the Customer shall destroy the Product and any materials or data created using the Product. In order to extend the use of the Product and derived materials or data, the Customer shall license the Product's technology from LKB.

3. Ordering and Payment

Purchase orders are submitted via email or through LKB's website at www.lockandkeybiosciences.com. Pre-payment is mandatory to place a purchase order. Purchase orders become binding only upon LKB's acceptance, which is communicated through email. Orders can be cancelled before Product shipment, subject to agreement with LKB. Unless otherwise specified, Product prices are as indicated by LKB on the company's website at the time of order placement. Prices may be subject to change based on various factors, including material costs, regulations, and transportation.

4. Product Purchase Limitations

The Products provided by LKB for commercial purposes are primarily intended for technology testing and evaluation, and they are subject to specific supply limitations. LKB's supply to each customer is restricted to a maximum of three Products, whether they are of identical or different types. LKB will only supply more than one Kit to a given Customer to facilitate testing in several locations, or to enable the evaluation of different technologies. The continued use of LKB technologies beyond this limit requires the execution of a licensing agreement. In extraordinary circumstances, deviations from this limitation may be considered by LKB following the submission of a formal request. LKB reserves the right to refuse the sale of ProteoShell Kits to prospective customers who are currently participating or have participated in a technology testing agreement for the ProteoShell System.

5. Delivery Time

LKB will dispatch Products within five business days from the order placement except otherwise announced. LKB is not responsible for delivery delays resulting from the shipping company or other external factors.

6. Product Acceptance and Return

Upon delivery, the Customer must inspect the integrity of the Products and may request a free replacement for any Product damaged during shipping within forty-eight hours. Product components displaying signs of decay before their expiration date will be replaced at no cost, except when mishandling by the Customer is the cause, as proper handling procedures are indicated in the Product labels and/or instruction manual.

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