For Beginners: The ProteoShell Kit I
Our starter Kit contains everything you need to express your proteins using the ProteoShell system.
The ProteoShell Kit I is designed to boost recombinant protein expression in E. coli. It is simple, fast, and effective. You will only require common laboratory equipment and skills to use it. Key features: •By inserting your gene into the pPS-I plasmid, your protein is merged N-terminally with ProteoShell •ProteoShells are expressed in T7 E. coli strains, e.g. BL21 (DE3) and derivatives •Induction is regulated with lactose / IPTG •Simple isolation of ProteoShells via selective precipitation followed by resolubilization •Release of the protein of interest using TEV protease (not included) •Optimal results for non-membrane proteins (or soluble domains of membrane proteins) with domains > 50KDa Kit contents: •pPS-I-(a) expression plasmid •Lysis Buffer •Harvest Buffer •4x Washing Buffer •4x Reconstitution Buffer The Kit is offered with reagents sufficient for 10 or 50 protein preparations. A preparation is defined as the processing of 10 g of freshly harvested cells, although the procedure is readily adapted to any desired amount.
For Advanced Users: The ProteoShell Kit I plus
Our expert users choose this Kit to tune the amount of protein of interest contained in each ProteoShell. Ideal for very small or very large proteins, and slow-translating sequences.
The ProteoShell Kit I is designed to boost recombinant protein expression in E. coli. It is simple, fast, and effective. You will only require common laboratory equipment and skills to use it. Key features: •By inserting your gene into the pPS-I plasmid, your protein is merged N-terminally with ProteoShell •Choose among the three pPS-I plasmids (a, b, and c) to tune the amount of protein of interest contained in each ProteoShell •ProteoShells are expressed in T7 E. coli strains, e.g. BL21 (DE3) and derivatives •Induction is regulated with lactose / IPTG •Simple isolation of ProteoShells via selective precipitation followed by resolubilization •Release of the protein of interest using TEV protease (not included) •Optimal results for non-membrane proteins (or soluble domains of membrane proteins) with domains > 50KDa Kit contents: •Expression plasmids pPS-I-(a), pPS-I-(b), and pPS-I-(c) •Lysis Buffer •Harvest Buffer •4x Washing Buffer •4x Reconstitution Buffer The Kit is offered with reagents sufficient for 10 or 50 protein preparations. A preparation is defined as the processing of 10 g of freshly harvested cells, although the procedure is readily adapted to any desired amount.
For Industrial Customers and Partners: The ProteoShell Kit I developer package
The ProteoShell Technology is applicable to the industrial production of proteins. To this end, we offer you the opportunity to develop your own custom ProteoShell applications under license of Lock and Key Biosciences. Contact us for more information.
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