Spending too much time troubleshooting
protein expression and purification?
You're not alone.
Overcome your expression challenges, and much more, with the ProteoShell System
ProteoShells are separated in seconds from cleared cell lysate upon addition of our proprietary Harvest Buffer. The resulting ProteoShell Solid is readily filtered/centrifuged, washed, and redissolved into purified ProteoShells
Tame defiant proteins through molecular organization
Protein of Interest
(POI)
pPS plasmid encoding POI
ProteoShells containing POI
POI released by protease clevage
(when desired)
Life science companies require a continuous stream of novel recombinant proteins for their research and development endeavors. However, many proteins pose challenges with conventional methodologies, resulting in costly setbacks and potential project failures. Common obstacles include aggregation, misfolding, toxicity, and degradation, often arising from uncontrolled interactions of the proteins with their molecular neighbors.
To circumvent these issues, molecular tools are imperative for protein scientists to systematically organize, encage, and package proteins immediately upon expression. The ProteoShell System capitalizes on these principles to facilitate the efficient production and purification of otherwise unattainable proteins. Within the ProteoShell, your proteins will be optimally posed and protected to excel in their applications.
One technology, endless opportunities
FAST-TRACK
CHALLENGING PROTEIN
EXPRESSION
The ProteoShell expedites the expression of proteins in E. coli by enclosing them in a protective cage during the expression process, preventing detrimental interactions and promoting stability. This approach has demonstrated success across a diverse array of proteins plagued by various expression challenges
PROTEIN EXPRESSION
MADE FAST AND EASY
Purifying ProteoShells is achieved within minutes without the need for chromatographic columns or costly beads. Utilizing our exclusive set of buffers, ProteoShells form a solid readily separable by filtration or centrifugation. After washing, the solid can be used directly or fully redissolved. When desired, proteins of interest and ProteoShells are detached by protease cleavage and readily separated.
HARNESS THE POWER OF PROTEIN
ORGANIZATION
The ProteoShell serves as a safeguarding and organizing vessel for diverse protein applications. Each ProteoShell has the capability to cluster different proteins, fostering synergistic effects in electron transfer reactions, cofactor regeneration, and other enzymatic cascades. As carriers for catalysts, ProteoShells can be effortlessly separated and recycled through microfiltration.
Enzyme catalysis in a protecting vessel
Enhanced protein-protein interactions
Synergistic
reaction
cascades
Xavier Garrabou Pi is the founder of Lock and Key Biosciences GmbH a spin-off from ETH Zürich (Switzerland). He is a chemist with more than fifteen years of experience in biocatalysis and protein engineering. His contributions are reported in several high-impact publications and patents.
